Molecular characterization of the Mycobacterium tuberculosis complex (MTC) of raw milk from selected dairy farms in the Eastern Cape

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dc.contributor.advisorGreen, Ezekielen
dc.contributor.advisorNdip, Rolanden
dc.contributor.authorKomani, Nosiphiwoen
dc.date.accessioned2026-08-12T23:25:37Z
dc.date.available2026-08-12T23:25:37Z
dc.date.issued2013en
dc.description.abstractTuberculosis (TB) is an ancient infectious disease that has been infecting different populations around the globe and it has also been considered as one of the most successful human and animal disease. TB found in animals such as cattle and other known bovids is known as bovine tuberculosis. Bovine tuberculosis (BTB) is an infectious disease found in cattle mainly caused by Mycobacterium bovis. M. bovis is a member of the Mycobacterium tuberculosis complex (MTC) together with M. tuberculosis, M. africanum, and M. canetti where the natural host is humans; whereas M. caprae, M. microti and M. pinnipedii usually have animals as their natural host. In this study the molecular characterization of the MTC from cow milk in the Eastern Cape was investigated. One hundred and twenty samples (40 ml each) were collected from three dairy farms in the Eastern Cape, South Africa. These samples were processed using a modified Petroff decontamination method. Sample processing was followed by DNA isolation using a Zymo Bacterial/Fungal DNA Kit and the amplification and detection of the MTC was done using the Seeplex MTB Nested ACE assay. The drug susceptibility tests were done using GenoTypeMTBDRplus assay which detects mutations and resistance to INH (isoniazid) and RMP (rifampicin). The milk isolates were further analyzed using a spoligotyping method which is based on the PCR amplification of a highly polymorphic direct repeat locus in the M. tuberculosis genome which detects and types the MTC. A percentage of 20.8 % samples were found to be positive for MTC using the Seeplex MTB Nested ACE assay. There were 42.1 % samples that were resistant to both INH and RMP with the rest sensitive to either INH or RMP. The spoligotyping method showed that 78.3 % samples resembled Family 33 strains and the rest (21.7 %) resembled a spoligotyping signature known to be that of M.africanum. Both these strains belong to the Ancestral lineage with Indo-Oceanic and West Africa 2 lineage. The outcomes of our study showed that molecular methods for detection of MTC can be applied directly on milk samples without the need for culturing.en
dc.formatxiii, 82 leaves :ill.; 30 cmen
dc.formatpdfen
dc.identifier.citationKomani, Nosiphiwo. 2013en
dc.identifier.othervital:11276en
dc.identifier.otherhttp://hdl.handle.net/10353/d1013157en
dc.identifier.otherMycobacterium tuberculosis -- South Africa -- Eastern Capeen
dc.identifier.otherMilk -- South Africa -- Eastern Capeen
dc.identifier.otherDairy farms -- South Africa -- Eastern Capeen
dc.identifier.otherDairy farming -- South Africa -- Eastern Capeen
dc.identifier.otherTuberculosis in cattle -- South Africa -- Eastern Capeen
dc.identifier.urihttp://hdl.handle.net/20.500.11837/4537
dc.language.isoEnglishen
dc.publisherUniversity of Fort Hareen
dc.publisherFaculty of Science & Agricultureen
dc.rightsUniversity of Fort Hareen
dc.subjectMycobacterium tuberculosis -- South Africa -- Eastern Capeen
dc.subjectMilk -- South Africa -- Eastern Capeen
dc.subjectDairy farms -- South Africa -- Eastern Capeen
dc.subjectDairy farming -- South Africa -- Eastern Capeen
dc.subjectTuberculosis in cattle -- South Africa -- Eastern Capeen
dc.titleMolecular characterization of the Mycobacterium tuberculosis complex (MTC) of raw milk from selected dairy farms in the Eastern Capeen
dc.title.alternativeMolecular characterization of the Mycobacterium tuberculosis complex (MTC) of raw milk from selected dairy farms in the Eastern Capeen
dc.typeThesisen
dc.typeMastersen
dc.typeMSc (Microbiology)en

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