Prevalence and antibiotic resistance determinants of Escherichia coli pathotypes obtained from raw milk in two farms from the Eastern Cape, South Africa: public health implications

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dc.contributor.advisorGreen, Een
dc.contributor.advisorNdip, N Ren
dc.contributor.advisorOkoh, Anthony, Ien
dc.contributor.authorCaine, Lesley-Anneen
dc.date.accessioned2026-08-12T23:25:37Z
dc.date.available2026-08-12T23:25:37Z
dc.date.issued2013en
dc.description.abstractMilk quality continues to be a topic of intense debate in the dairy industry, medical and public health communities. Production of maximum quantities of high-quality milk is an important goal of every dairy operation. High-quality milk must contain a low number of somatic cells and low bacteria count, and must be free of human pathogens and antibiotic residues. The objective of this study was to determine the prevalence of E. coli in unpasteurized milk recovered from Middledrift and Fort Hare dairy. In this study 400 milk samples were collected from two commercial farms (Middledrift and Fort Hare) in the Eastern Cape, South Africa, 200 raw milk samples from each farm. Samples were cultured on violet red bile mug-agar (VRB-MUG Agar) and incubated at 37ÂșC for 24 hours and preliminary identified by Gram stain and catalase test. Isolates that were Gram negative and catalase positive were screened for a marker of E. coli uidA gene using PCR assays. Middledrift dairy farm had 50 (25%) E. coli isolated from raw milk and Fort Hare farm showed 37 (18.5%) E. coli present in the milk samples. The presence of E. coli found in the milk samples points to the fact that fecal contamination was unavoidable and traditional practices are likely to contribute to the contamination of the milk and proliferation of the microorganisms.en
dc.format146 leaves; 30 cmen
dc.formatpdfen
dc.identifier.citationCaine, Lesley-Anne. 2013en
dc.identifier.othervital:11277en
dc.identifier.otherhttp://hdl.handle.net/10353/d1015525en
dc.identifier.otherRaw Milk -- Escherichia colien
dc.identifier.otherPolymerase -- Chain Reaction (PCR)en
dc.identifier.urihttp://hdl.handle.net/20.500.11837/4538
dc.language.isoEnglishen
dc.publisherUniversity of Fort Hareen
dc.publisherFaculty of Science & Agricultureen
dc.rightsUniversity of Fort Hareen
dc.subjectRaw Milk -- Escherichia colien
dc.subjectPolymerase -- Chain Reaction (PCR)en
dc.titlePrevalence and antibiotic resistance determinants of Escherichia coli pathotypes obtained from raw milk in two farms from the Eastern Cape, South Africa: public health implicationsen
dc.title.alternativePrevalence and antibiotic resistance determinants of Escherichia coli pathotypes obtained from raw milk in two farms from the Eastern Cape, South Africa: public health implicationsen
dc.typeThesisen
dc.typeMastersen
dc.typeMSc (Microbiology)en

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