Assessment of the anti-Listerial properties of Garcinia kola (Heckel) seeds

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dc.contributor.advisorOkoh, Anthony Ien
dc.contributor.advisorMayekiso, Ben
dc.contributor.advisorBuwa, Len
dc.contributor.authorPenduka, Dambudzoen
dc.date.accessioned2026-08-12T23:25:37Z
dc.date.available2026-08-12T23:25:37Z
dc.date.issued2014en
dc.description.abstractA follow-up of traditional medicinal plants uses is an important tool in highlighting their therapeutic potentials, as they have been found to be a source of a wide range of bioactive compounds that can be used as base compounds for new pharmaceutical drugs. This study therefore focuses on assessing the anti-Listerial properties of the seeds of Garcinia kola (Heckel) plant, which is a traditional medicinal plant of west and central African origin, and was and is still used to traditionally treat several ailments. Four different solvents crude extracts of the seeds were assessed for their anti-Listerial activities in-vitro, against a panel of 42 Listeria bacteria, which included Listeria monocytogenes, Listeria ivanovii and Listeria grayi species. At 10 mg/ml concentration the aqueous extract had activity against 29% of the test isolates while the other three crude extracts namely dichloromethane, n-hexane and the methanol extracts had activity against 45% of the test bacteria. The minimum inhibitory concentration (MIC) ranges of the extracts were 0.079-0.313 mg/ml for the dichloromethane extract; 0.079-0.625 mg/ml for the n-hexane extract; 0.157-0.625 mg/ml for the methanol extract; and 10->10 mg/ml for the aqueous extract. The minimum bactericidal concentration (MBC) ranges of the extracts were 0.625–10 mg/ml for both the n-hexane and the dichloromethane extract; 5-10 mg/ml for the methanol extract; and those for the aqueous extract were above 10 mg/ml against all the susceptible Listeria isolates. The rate of kill analysis was then determined for the three most active crude extracts that is excluding the aqueous extract and it was assessed against four representative Listeria species namely L. monocytogenes (LAL 8), L. grayi (LAL 15), L. ivanovii (LEL 30) and L. ivanovii (LEL 18). All the three extracts showed a general trend of being concentration and time dependent in their rate of kill profiles such that most bacteria cells were killed at the highest test concentration of 4× MIC value after the maximum exposure time of 2 h. The n-hexane, dichloromethane and methanol extracts were bactericidal against 4, 3 and 1 isolates out of the four test Listeria isolates respectively.en
dc.format225 leaves; 30 cmen
dc.formatpdfen
dc.identifier.citationPenduka, Dambudzo. 2014en
dc.identifier.othervital:11278en
dc.identifier.otherhttp://hdl.handle.net/10353/d1015527en
dc.identifier.otherMicrobial sensitivity testsen
dc.identifier.otherGarciniaen
dc.identifier.otherMedicinal plantsen
dc.identifier.otherTraditional medicineen
dc.identifier.urihttp://hdl.handle.net/20.500.11837/4539
dc.language.isoEnglishen
dc.publisherUniversity of Fort Hareen
dc.publisherFaculty of Science & Agricultureen
dc.rightsUniversity of Fort Hareen
dc.subjectMicrobial sensitivity testsen
dc.subjectGarciniaen
dc.subjectMedicinal plantsen
dc.subjectTraditional medicineen
dc.titleAssessment of the anti-Listerial properties of Garcinia kola (Heckel) seedsen
dc.title.alternativeAssessment of the anti-Listerial properties of Garcinia kola (Heckel) seedsen
dc.typeThesisen
dc.typeDoctoralen
dc.typePhD (Microbiology)en

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