Inhibitory potential of honey on the enzymatic activity of Helicobacter pylori urease

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dc.contributor.advisorClarke, Aen
dc.contributor.advisorBradley, Gen
dc.contributor.advisorNdip, Ren
dc.contributor.authorMatongo, Fredricken
dc.date.accessioned2026-08-12T23:25:34Z
dc.date.available2026-08-12T23:25:34Z
dc.date.issued2012en
dc.description.abstractUrease of Helicobacter pylori is an important virulence factor implicated in the pathogenesis of many clinical conditions, such as chronic gastritis, peptic ulceration, and gastric cancer. Many urease inhibitors have been discovered, like phosphorodiamidates, hydroxamic acid derivatives, and imidazoles. Despite good activities at the enzyme level and excellent kinetic properties most of them have not been used as therapeutic agents in vivo because of their side effects, toxicity and instability. This has led to much attention to focus on exploring the novel urease inhibitory activities of natural products because of their low toxicity and good bioavailability. Honey, a natural product has been used in folk medicine due to its antitumor, antioxidant, antimicrobial and anti-inflammatory properties. The aims of this study were to isolate, characterise, purify urease produced by H. pylori and investigate the inhibitory effects of solvent honey extracts on its enzymatic activity. Urease was found to be both surface-associated and cytoplasmic. Maximum cytoplasmic urease activity was found to occur after 72 hr whereas maximum extracellular urease activities were found to occur after 96 hr. Characterization of the crude cytoplasmic urease revealed optimal activity at a pH of 7.5 and temperature of 40°C. The kinetic parameters Vmax and Km were 45.32 U ml-1 and 61.11 mM respectively.The honey extracts inhibited the activity of the crude urease in a concentration dependent manner. The Lineweaver-Burk plots indicated a non-competitive type of inhibition against H. pylori urease. The two honey extracts gave promising inhibitory activities against urease of H. pylori. Thus the results of this study delineates that inhibition of urease can ease development in therapeutic and preventative approaches based on the enzymatic activity of this Helicobacter protein.en
dc.format134 leaves; 30 cmen
dc.formatpdfen
dc.identifier.citationMatongo, Fredrick. 2012en
dc.identifier.othervital:11253en
dc.identifier.otherhttp://hdl.handle.net/10353/431en
dc.identifier.otherHoneyen
dc.identifier.otherHelicobacter pylori infectionsen
dc.identifier.otherEnzyme inhibitorsen
dc.identifier.otherTraditional medicineen
dc.identifier.otherAntifungal agentsen
dc.identifier.urihttp://hdl.handle.net/20.500.11837/4514
dc.language.isoEnglishen
dc.publisherUniversity of Fort Hareen
dc.publisherFaculty of Science & Agricultureen
dc.rightsUniversity of Fort Hareen
dc.subjectHoneyen
dc.subjectHelicobacter pylori infectionsen
dc.subjectEnzyme inhibitorsen
dc.subjectTraditional medicineen
dc.subjectAntifungal agentsen
dc.titleInhibitory potential of honey on the enzymatic activity of Helicobacter pylori ureaseen
dc.title.alternativeInhibitory potential of honey on the enzymatic activity of Helicobacter pylori ureaseen
dc.typeThesisen
dc.typeMastersen
dc.typeMSc (Biochemistry)en

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