Antibacterial properties of the methanol extract of helichrysum pedunculatum

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dc.contributor.advisorOkoh, Anthony Ien
dc.contributor.advisorAfolayan, A Jen
dc.contributor.authorNcube, Nqobile Sen
dc.date.accessioned2026-08-12T23:25:32Z
dc.date.available2026-08-12T23:25:32Z
dc.date.issued2008en
dc.description.abstractThe methanol extract of Helichrisum pedunculatum was screened for antimicrobial activity up to a concentration of 5 mg/ml using the agar dilution technique. A number of test bacterial isolates, comprising both Gram negative and Gram positive organisms were susceptible to the crude extract of the plant. The minimum inhibitory concentrations (MICs) of the extract ranged between 1 and 5 mg/ml for the susceptible organisms. The MICs of the selected antibiotics, chloramphenicol and penicillin, ranged between 2 and 4 mg/L, and 2 and 32 mg/L respectively against Bacillus cereus, Proteus vulgaris and Staphylococcus aureus OKOH1. Bactericidal activity was determined by the time kill assay. The methanol extract of the plant was not bactericidal at 1 × MIC for B. cereus, P. vulgaris and Staph. aureus OKOH1. At 2 × MIC the extract was bacteriostatic against B. cereus but bactericidal against P. vulgaris and Staph. aureus OKOH1. Combination studies were done at 1/2 × MIC, 1 × MIC and 2 × MIC of the plant extract with 1 × MIC of the antibiotics. Combinations of the plant extract and chloramphenicol resulted in mostly indifferent interactions against P. vulgaris and Staph. aureus OKOH1 but synergistic interactions at higher concentration of the plant extract for B. cereus. Penicillin combinations gave synergistic interactions at lower concentrations of the plant for P.vulgaris and Staph. aureus OKOH1 but was mostly indifferent for B. cereus.en
dc.formatvii, 69 leaves; 30 cmen
dc.formatpdfen
dc.identifier.citationNcube, Nqobile S. 2008en
dc.identifier.othervital:11241en
dc.identifier.otherhttp://hdl.handle.net/10353/461en
dc.identifier.otherMedicinal plantsen
dc.identifier.otherMethanolen
dc.identifier.otherHelichrysumen
dc.identifier.urihttp://hdl.handle.net/20.500.11837/4502
dc.language.isoEnglishen
dc.publisherUniversity of Fort Hareen
dc.publisherFaculty of Science & Agricultureen
dc.rightsUniversity of Fort Hareen
dc.subjectMedicinal plantsen
dc.subjectMethanolen
dc.subjectHelichrysumen
dc.titleAntibacterial properties of the methanol extract of helichrysum pedunculatumen
dc.title.alternativeAntibacterial properties of the methanol extract of helichrysum pedunculatumen
dc.typeThesisen
dc.typeMastersen
dc.typeMSc (Microbiology)en

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