In-vitro anti-vibrio activities of crude extracts of Garcinia Kola seeds

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dc.contributor.advisorOkoh, Anthony Ien
dc.contributor.authorPenduka, Dambudzoen
dc.date.accessioned2026-08-12T23:25:35Z
dc.date.available2026-08-12T23:25:35Z
dc.date.issued2011en
dc.description.abstractThe n-Hexane, dichloromethane, methanol and aqueous crude extracts of Garcinia kola (Heckel) seeds were screened for their anti-Vibrio activities against 50 Vibrio bacteria isolated from wastewater final effluents. The 50 isolates consisted of different Vibrio species namely V. fluvialis (14), V. vulnificus (12), V. parahaemolyticus (12), V. metschnikovii (3) and 9 others unidentified to the specie level. The n-Hexane, dichloromethane and methanol extracts had activities against 16 (32 percent) of the Vibrio isolates, while the aqueous extracts had activities against 12 (24 percent) all at a screening concentration of 10 mg/ml. The minimum inhibitory concentrations (MICs) were 0.313-0.625 mg/ml, 0.313-0.625 mg/ml, 0.313-2.5 mg/ml and 10 mg/ml for n-Hexane, dichloromethane, methanol and aqueous extracts respectively. Rate of kill studies were carried out against three different Vibrio species namely V. vulnificus (AL042), V. parahaemolyticus (AL049) and V. fluvialis ( AL040) using the n-Hexane, dichloromethane and methanol extracts at 1× to 4 × MICs and 2 hour exposure. About 96.3 percent, 82.2 percent, and 78.1 percent (V. fluvialis AL040); 92.6 percent, 87.8 percent and 68.9 percent (V. parahaemolyticus AL049); and 91.6 percent, 64.4 percent, 60 percent (V. vulnificus AL042) of the bacteria were killed by the crude n-Hexane, dichloromethane and methanol extracts respectively after 2 hour exposure time at 4× MIC. The patterns of activity were bacteriostatic, with the n-Hexane extracts being most effective in activity. We conclude that the Garcinia kola seeds have promise in the treatment and management of infections caused by Vibrio species.en
dc.format117 leaves; 30 cmen
dc.formatpdfen
dc.identifier.citationPenduka, Dambudzo. 2011en
dc.identifier.othervital:11256en
dc.identifier.otherhttp://hdl.handle.net/10353/405en
dc.identifier.otherMicrobial sensitivity testsen
dc.identifier.otherDrug resistance in microorganismsen
dc.identifier.otherAntibioticsen
dc.identifier.otherGarciniaen
dc.identifier.otherMedicinal plantsen
dc.identifier.urihttp://hdl.handle.net/20.500.11837/4517
dc.language.isoEnglishen
dc.publisherUniversity of Fort Hareen
dc.publisherFaculty of Science & Agricultureen
dc.rightsUniversity of Fort Hareen
dc.subjectMicrobial sensitivity testsen
dc.subjectDrug resistance in microorganismsen
dc.subjectAntibioticsen
dc.subjectGarciniaen
dc.subjectMedicinal plantsen
dc.titleIn-vitro anti-vibrio activities of crude extracts of Garcinia Kola seedsen
dc.title.alternativeIn-vitro anti-vibrio activities of crude extracts of Garcinia Kola seedsen
dc.typeThesisen
dc.typeMastersen
dc.typeMSc (Microbiology)en

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