Bioactivity and phytochemical analysis of Hydnora Africana on some selected bacterial pathogens

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dc.contributor.advisorOkoh, Anthony Ien
dc.contributor.authorNethathe, Bono Biancaen
dc.date.accessioned2026-08-12T23:25:33Z
dc.date.available2026-08-12T23:25:33Z
dc.date.issued2011en
dc.description.abstractAbstract Medicinal plants have been for long remedies for human diseases because they contain components of therapeutic value. The growing problem of antibiotic resistance by organisms demands the search for novel compounds from plant based sources. The present study was aimed at evaluating the bioactivity and phytochemical analysis of Hydnora africana on clinical and standard strains of Helicobacter pylori (PE 252C and ATCC 43526), Aeromonas hydrophila ATCC 35654, and Staphylococcus aureus NCT 6571 in an effort to identify potential sources of cheap starting materials for the synthesis of new drugs against these strains. Ethyl acetate, acetone, ethanol, methanol, and water crude extracts of H. africana were screened for activity against the test organisms using the agar well diffusion assay. The Minimum Inhibitory Concentration (MIC50) and Minimum Bactericidal Concentration (MBC) of the most potent extracts were determined by the microdilution method, followed by qualitative phytochemical analysis. Results were analyzed statistically by ANOVA one - way test. Different concentrations (200,100, 50mg/mL) of the methanol, acetone, ethanol and ethyl acetate extracts showed activity against S. aureus and A. hydrophila while for H. pylori, only methanol and ethyl acetate extracts were active; water showed no activity for all studied bacterial pathogens. Mean zone diameter of inhibition which ranged from 0-22mm were observed for all test bacterial pathogens and 14-17mm for ciprofloxacin. The activity of methanol and ethyl acetate extracts were statistically significant (P< 0.05) compared to all the other extracts. MIC50 and MBC ranged from 0.078 – 2.5mg/mL, 0.78-25mg/mL respectively for all tested bacterial pathogens. For ciprofloxacin, the MIC50 and MBC ranged from 0.00976 – 0.078mg/mL and 0.098– 0.78mg/mL respectively. There was no statistically significant difference between extracts (methanol, acetone, ethanol, ethyl acetate) and the control antibiotic (ciprofloxacin) (P> 0.05). Qualitative phytochemical analysis confirmed the presence of alkaloids, saponins, steroids, tannins and flavonoids in the methanol, acetone,ethanol and ethyl acetate extracts. The results demonstrate that H. africana may contain compounds with therapeutic potentials which can be lead molecules for semi-synthesis of new drugs.en
dc.formatxii, 90 leaves; 30 cmen
dc.formatpdfen
dc.identifier.citationNethathe, Bono Bianca. 2011en
dc.identifier.othervital:11247en
dc.identifier.otherhttp://hdl.handle.net/10353/d1001063en
dc.identifier.otherHelicobacter pylorien
dc.identifier.otherMedicinal plants -- South Africa -- Eastern Capeen
dc.identifier.otherMicrobial sensitivity testsen
dc.identifier.otherHerbs -- Therapeutic use -- South Africa -- Eastern Capeen
dc.identifier.otherPlants -- Analysisen
dc.identifier.otherStaphylococcus aureusen
dc.identifier.otherAeromonas hydrophilaen
dc.identifier.otherDrug resistance in microorganismsen
dc.identifier.otherPlant-pathogen relationshipsen
dc.identifier.urihttp://hdl.handle.net/20.500.11837/4508
dc.language.isoEnglishen
dc.publisherUniversity of Fort Hareen
dc.publisherFaculty of Science & Agricultureen
dc.rightsUniversity of Fort Hareen
dc.subjectHelicobacter pylorien
dc.subjectMedicinal plants -- South Africa -- Eastern Capeen
dc.subjectMicrobial sensitivity testsen
dc.subjectHerbs -- Therapeutic use -- South Africa -- Eastern Capeen
dc.subjectPlants -- Analysisen
dc.subjectStaphylococcus aureusen
dc.subjectAeromonas hydrophilaen
dc.subjectDrug resistance in microorganismsen
dc.subjectPlant-pathogen relationshipsen
dc.titleBioactivity and phytochemical analysis of Hydnora Africana on some selected bacterial pathogensen
dc.title.alternativeBioactivity and phytochemical analysis of Hydnora Africana on some selected bacterial pathogensen
dc.typeThesisen
dc.typeMastersen
dc.typeMSc (Microbiology)en

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