Evaluation of the possible application of cowpea genotypes in the farming systems of the Eastern Cape Province, South Africa

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dc.contributor.advisorBradley, Gen
dc.contributor.advisorOkoh, Anthony Ien
dc.contributor.advisorLewu, Fen
dc.contributor.authorAdeyemi, Samson Adebowaleen
dc.date.accessioned2026-08-12T23:25:37Z
dc.date.available2026-08-12T23:25:37Z
dc.date.issued2012en
dc.description.abstractCharacterization studies on the genetic diversity among cultivated cowpea (Vigna unguiculata (L.) varieties are valuable tools to optimize the use of available genetic resources by farmers, local communities, researchers and breeders. Eight cowpea (Vigna unguiculata (L.) genotypes ( Vegetable cowpea, Ivory grey, Okhalweni, Fahari, Fahari dark, 97K-1069-8, IT93K-73h, and 129-3) were subjected to molecular, morphological and agronomical characterization. DNA amplification fingerprinting markers were used to evaluate the genetic diversity among the eight genotypes. Nine random arbitrary primers were used to screen the eight genotypes to assess their ability to reveal polymorphisms in cowpea, and seven of them were selected for use in characterizing the total sample. A total of 43 bands were generated which are all polymorphic. On the average, the primers generated a total of 6.1 polymorphic bands. The resulting data-matrix included 43 analysed bands with a total of 344 characters. Neighbour joining analysis was used to generate the dendrogram, clustering the genotypes into two groups at an agglomerate coefficient of 0.30 irrespective of their geographical origins. The results also showed the presence of significant differences in morphological and quality traits among the genotypes. Fahari yielded the highest concentration of crude protein (46.51 mg/mg dry leaf) while Vegetable cowpea yielded the lowest (24.41 mg/mg dry leaf). The influence of manure was also found to be effective by increasing the crude protein content of the genotypes as shown by Fahari dark with an average of 53.53 mg/mg dry leaf as opposed to 39.85 mg/mg dry leaf without manure application. Although some small clusters grouped accessions of the same growth habits, a general lack of agreement between clustering and morphological features was observed. It can therefore be concluded that the significant differences between the molecular genetic analysis using DAF-PCR markers, morphologic characters and yield traits can be important tools to identify and discriminates the different cowpea genotypes.en
dc.format91 leaves; 30 cmen
dc.formatpdfen
dc.identifier.citationAdeyemi, Samson Adebowale. 2012en
dc.identifier.othervital:11274en
dc.identifier.otherhttp://hdl.handle.net/10353/d1007539en
dc.identifier.otherCowpea -- South Africa -- Eastern Capeen
dc.identifier.otherCowpeaen
dc.identifier.otherPlant diversityen
dc.identifier.otherCowpea -- Geneticsen
dc.identifier.otherDNA fingerprinting of plantsen
dc.identifier.urihttp://hdl.handle.net/20.500.11837/4535
dc.language.isoEnglishen
dc.publisherUniversity of Fort Hareen
dc.publisherFaculty of Science & Agricultureen
dc.rightsUniversity of Fort Hareen
dc.subjectCowpea -- South Africa -- Eastern Capeen
dc.subjectCowpeaen
dc.subjectPlant diversityen
dc.subjectCowpea -- Geneticsen
dc.subjectDNA fingerprinting of plantsen
dc.titleEvaluation of the possible application of cowpea genotypes in the farming systems of the Eastern Cape Province, South Africaen
dc.title.alternativeEvaluation of the possible application of cowpea genotypes in the farming systems of the Eastern Cape Province, South Africaen
dc.typeThesisen
dc.typeMastersen
dc.typeMSc (Biochemistry)en

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